负载有灵芝多糖的ROS反应果胶/PVA水凝胶通过偏振巨噬细胞对M2表型加速糖尿病伤口愈合

Diabetic chronic wound healing is hindered by excessive reactive oxygen species (ROS) and dysregulated inflammation. To address this challenge, we developed a multifunctional hydrogel based on natural polymer. Specifically, an ROS-responsive hydrogel was fabricated via dynamic boronate ester bonds between phenylboronic acid-functionalized pectin (Pe-PBA) and poly(vinyl alcohol) (PVA), then loaded with Ganoderma atrum polysaccharide (PSG), termed Pe/PVA@PSG. The obtained hydrogel exhibited excellent mechanical properties and self-healing ability, enabling efficient PSG release triggered in response to high-glucose and -ROS conditions. In vitro, it showed excellent biocompatibility, significantly facilitated fibroblast proliferation and migration, and suppressed ROS accumulation and macrophage inflammatory responses. In a diabetic wound model, Pe/PVA@PSG markedly accelerated wound closure, achieving 93.42% closure on day 14, which enhanced collagen deposition and skin appendage regeneration, elevated IL-10, upregulated PD-L1, and drove macrophage polarization toward the M2 phenotype. Overall, the Pe/PVA@PSG hydrogel provides an innovative strategy for chronic diabetic wound management.