黄精中的高异黄酮、生物碱和倍半萜及其免疫调节作用

Polygonatum cyrtonema (Huang jing) is widely used in both the food and traditional Chinese medicine industries. A chemical investigation of the rhizome of cultivated P. cyrtonema yielded six previously undescribed compoundsone norsesquiterpenoid (1), one pyridine alkaloid (2), and four homoisoflavanones (3a, 4a, 5a, 6a)together with 9 known compounds (3b, 4b, 5b, 6b, 7-11). Their structures were established on the basis of extensive spectroscopic data and quantum chemical calculations, including NMR, HRESIMS, and calculated ECD. The enantiomers of the C-6-methylated homoisoflavanones (3a/3b-6a/6b) were successfully isolated via chiral HPLC. All compounds were evaluated for their immunomodulatory activity using RAW264.7 macrophages at concentrations ranging from 3.125 to 25 μM. Only compounds 8 and 11 enhanced the proliferation and phagocytosis of RAW264.7 macrophages and upregulated the secretion of NO, IL-6, and TNF-α.