- 期刊Food chemistry（Food Chem）
- 发表时间2026-10-15
- 作者Xu S, Wei X, Fang D, Tian X, Zhu D, Zhang Y, Ma L, Han H, Song G
- 卷期页码V532(Pt 2) : 151645
- DOI10.1016/j.foodchem.2026.151645
- PMID42892036
摘要机译
本研究开发了一种pH转移辅助组装策略来构建可溶性棉籽分离蛋白（CPI）-虫胶复合物（CPS），在蛋白质功能和营养传递方面取得了显着的改善。首先，溶解度得到了显著提高：CPS系统在中性pH下达到了94.5%的接近完全溶解度，而天然CPI仅为6.8%。
此外，通过多尺度方法系统地解释了潜在的增溶机制：理化表征证实虫胶对疏水区域的有效屏蔽；蛋白质组学揭示了更均匀和稳定的蛋白质状态；分子动力学模拟直观地表明虫胶分子选择性地修补关键的疏水残基，从而抑制聚集。
值得注意的是，CPS作为姜黄素递送载体表现出优异的性能，实现了75%的高封装效率（EE），并显着提高了姜黄素的光热稳定性、细胞摄取和抗迁移生物活性。这项工作为从植物蛋白质开发高性能营养载体提供了一个绿色、可扩展、多尺度的策略。
展开英文摘要原文
This study developed a pH-shifting assisted assembly strategy to construct soluble cottonseed protein isolate (CPI)-shellac complexes (CPS), achieving remarkable improvements in both protein functionality and nutraceutical delivery. First, a dramatic enhancement in solubility was accomplished: the CPS system reached a near-complete solubility of 94.5% at neutral pH, compared to only 6.8% for native CPI. Furthermore, the underlying solubilization mechanism was systematically deciphered through a multi-scale approach: physicochemical characterization confirmed effective shielding of hydrophobic regions by shellac; proteomics revealed a more homogeneous and stable protein state; and molecular dynamics simulations visually demonstrated that shellac molecules selectively patch key hydrophobic residues, thereby inhibiting aggregation. Notably, the CPS exhibited excellent performance as a curcumin delivery vehicle, achieving a high encapsulation efficiency (EE) of 75% and significantly improving the photothermal stability, cellular uptake, and anti-migration bioactivity of curcumin. This work provides a green, scalable, multi-scale strategy for developing high-performance nutraceutical carriers from plant proteins.
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