LC-MS/MS测定药用和食用蒲公英中真菌毒素的方法的开发和验证

Dandelion (Taraxacum mongolicum Hand.-Mazz.) is a medicinal and edible herb widely used in traditional Chinese medicine; however, studies on mycotoxin contamination in dandelion remain limited. Herein, we developed a liquid chromatography-triple quadrupole-tandem mass spectrometry (LC-QqQ-MS/MS) method for the simultaneous quantification of five common mycotoxins: aflatoxin B1 (AFB1), deoxynivalenol (DON), fumonisin B1 (FB1), ochratoxin A (OTA), and zearalenone (ZEN) in dandelion. The mycotoxins were extracted from dandelion herb, cleaned up by solvent dilution, and analyzed in multiple reaction monitoring (MRM) mode on an LC-QqQ-MS/MS system. Key parameters were optimized to achieve the best performance, including chromatographic conditions, MRM ion pairs, extraction solvents, and sample cleanup methods. Method validation was conducted in terms of specificity, limit of determination, limit of quantitation, linearity, intraday and interday precision, and matrix effect. Finally, a total of 54 dandelion samples collected from different regions of China were analyzed using the developed method. AFB1 and FB1 were detected in all the dandelion samples, while OTA was found in 45 samples. DON was detected in nine samples from three regions, whereas ZEN was not detected in any of the samples. FB1 was found to be the most prevalent mycotoxin in the samples, ranging from 9.77 to 35.86 μg/kg, followed by AFB1 and OTA, indicating co-occurrence of mycotoxins in this herb.